Expression and role of RASSF1A gene in human gastric cancer / 上海第二医科大学学报

ABSTRACT

Objective To study the expression of RASSF1A gene in gastric cancer tissues and cell lines and to investigate its clinical roles. Methods Immunohistochemistry was employed to detect the expression of RASSF1A gene in 39 cases of human gastric cancer tissues and 18 cases of normal human gastric tissues, and RT-PCR was used to examine the expression of RASSF1A mRNA in 4 gastric cancer cell lines, normal gastric cell lines GES-1 and positive control Hela cells. Correlations between expression of RASSF1A gene and clinicopathological characteristics of gastric cancer were also analyzed. Results All the normal gastric tissues (18 cases) were stained with anti-RASSF1A antibody, and significantly lower expression of RASSF1A was found in the 39 cases of gastric cancer tissues (P