Effect of Daidzein intravitreal injection on optic nerve injury in rats / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 1378-1381, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-641970
ABSTRACT
AIM:
To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages.METHODS:
After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group ( 10μmol/L, 100μmol/L, 1000μmol/L ) and positive control group using rats nerve growth factor ( mNGF, 100ng/mL ). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups.RESULTS:
Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance (P<0. 05).CONCLUSION:
Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
International Eye Science
Ano de publicação:
2014
Tipo de documento:
Artigo
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