Virulence Factors and Genotyping of Vibrio parahaemolyticus in Sea Water and Clinical Isolates

ABSTRACT

Six strains of Vibrio parahemolyticus isolated from diarrheal patients and the 12 strains from sea water were serotyped and analyzed for biochemical characteristics, antibiotics sensitivity and detection of toxR, gyrB, tdh, and trh genes. Arbitrarily-primed polymerase chain reaction method were performed on the 6 strains from patients and the following results were obtained. 1. The Vibrio parahemolyticus isolated from patients were belonged to 5 different serotypes 04K8, 04KUT, 06 K18, 010K71 and 03K6, but those isolated from sea water were belonged to 5 different serotypes O1KUT, 02KUT, 03K45, 04K37 and OUTKUT. All strains explained have different serotypes depending on the different source, 2. Three serotype (04K8, 04KUT, 06K18) isolated from patients were positive for the urease hydrolysis, whereas only one strain of serotype O1KUT isolated from sea water was positive to the same. Furthermore, the serotype 06K18 (1 strain) was positive for the fermentation of dulcito1. Both toxR and gyrB genes were detected from all strains isolated. 3. As for control the 2 strains of serotype 03K6 and 6 strains isolated from patients, serotype 03K6 were resistant to oxacillin, penicillin, vancomycin. All strains were sensitive to chloramphenicol and tetracycline yet the antibiogram type showed 6 groups from I to VI. 4. DNA probe hybridization method was used to detect genes. The trh1 was detected both from serotype 04KUT and 06K18 isolated from patients and the trh2 was also detected from one strain from each 010K71 and O1KUT isolated from patients and sea-water respectively, The tdh gene only was detected from two strains of 03K6 isolated from patients of 1998. The tdh, trh 1 and trh2 were not detected from 7 strains out of 12 strains isolated from sea water whereas the production titer of TDH isolated from patients showed from 2048 times to 4096 times. 5. Four strains of the serotype 03K6 isolated from Korea, India and Japan as well as 3 strains from Korean patients were tested by AP-PCR to classify serotypes. As for its result the amplicon showed the same in the 4 strains of the serotype 03K6 whereas the four strains of different serotype from patients are so difference as to explain no inter- relations at all. The result explains that the serotype 03K6 is the same genes regardless from where it is isolated.