Quantitative determination of metoprolol by liquid chromatography mass spectrometry in rat plasma / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
;
(6): 543-545, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-642528
ABSTRACT
Objective:
To establish a highly sensitive, rapid and selective liquid chromatography mass spectrometry (LC-MS) method for the determination of metoprolol in rat plasma.Methods:
A simplified liquid-liquid extraction with acetidin was employed for the sample preparation. The separation was carried out on a Thermo ODS-C_(18)(5 μm,100 mm×2.1 mm).The mobile phase consisted of acetonitrile-methanol-water(20∶20∶60). Propranolol was used as the internal standard. The detection was performed on a liquid chromatography mass spectrometry by selected ion monitoring(SIM) scan mode electrospray ionization(ESI).Results andConclusion:
The range of calibration curve was 0.1-50 ng/ml and the limit of quantification was 0.1 ng/ml. The intra- and inter-day precision RSD was less than 15%.This method is sensitive, simple,rapid and suitable for the pharmacokinetic study of metoprolol.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Bulletin of The Academy of Military Medical Sciences
Ano de publicação:
2009
Tipo de documento:
Artigo
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