Effect of Ashi-antidiarrheal capsule on gastrointestinal hormones in blood plasma of thyroid hormone-induced diarrheic rats / 中国地方病学杂志

ABSTRACT

Objective Investigate the Asi-antidiarrheal capsule's effect on gastrointestinal horrnones in blood plasma of thyroid hormone-induced diarrheic(AbbreviationHyperthyroid Diarrhea.,D)rats.Methods One hundred and twenty SD male rats about 8 weeks old were randomly divided according to their constitution into control group of 10 rats and thyroid hormone-induced diarrheic group of 110 rats.The control group rats welle hvaged with isotonic Na chloride 1 ml/d.Thyroid tablets were made with isotonic Na chloride into 40 g/L susnl. The such solution with 1 ml/d was intragastrically administered to each rat in thyroid hormone-induced diardleic group.After three weeks,blood was sampled from vena caudalis of each rat.FT4 were then detected in blood semm.Fourty-two thyroid hormone-induced diarrheic rats were screened based on FT3 and FT4 level in blood serum, wet stool and body weisht.Fourty thyroid hormone-induced diarrheic rats were stochastically re-divided into 5 groups with 8 in each.The physiological saline with 1 ml/d was given to blank group,1.94 g·-kg-1·d-1 Berberine capsule to positive control group,and 0.63,1.26,2.52 g·kg-1·d-1 to low-dose,moderate-dose and high-dose groups respectivelv. Intragastric administration of each group continued for 7 days.Venous blood was centrifuged before and after administration and underwent radioimmunoassay to observe the effect of Ashi-antidiarrheal capsule on motillty (MTL),gastrin(GAS),somatostatin(SS),vasoactive intestinal polypeptide(VlP)in blood plasma of thyroid homlone- induced diarrheic rats.Results①Weight of thyroid hormone-induced diarrheic rats decreased[(344.0±12.9)g], FT3[(4,58 ±0.70)mol/L]and FT4[(23.44±4.40)mol/L]increased,and weight of wet stool[(17.4±3.2)g] increased.Compared to control group[(386.0±1.8)g,(2.08±0.10)mol/L,(10.18±2.00)mol/L,(9.1±0.6)g], there was a statistical significance(t=6.85,9.80,7.66,7.18,P<0.01).②After treatment,high-dose Ashi-antidiarrheal group[(80.54 ±3.80)ng/L]and positive control group[(90.63 ±9.99)ng/L]blood plasma MTL, compared to pre-therapy[(204.27±17.69),(187.79±13.32)ng/L]was decreased,there wag a statistical significance (t=8.60,4.57,P<0.01)③GAS contentshad respectively decreased comparedtopre-therapy[(192.75±11.80), (193.09±3.81),(190.60±9.31),(196.33±18.13)ng/L]in positive control group[(56.06 ±6.36)ng/L],low- dose group[(90.88±4.18)ng/L],midst-dose group[(75.64±7.09)ng/L]and hish-dose group[(44.32±3.72) ng/L],except for blank group.There Wag a statistical significance(t=15.27,7.62,13.43,13.22,all P<0.01).The intm-group difference of MTL,GAS and VIP level had statistic signifieances before and after the treatment(F= 166.68,1503.53,216.68,P<0.01).Conclusion Ashi-antidiarrheal capsule Can significantly lower the level of MTL and GAS in blood plasma。And raise the level of VIP.