Cloning and expression of dengue 2 virus NS1 gene in eukaryotic cells / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
;
(6): 5-8, 2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-642694
ABSTRACT
Objective:To investigate the correct expression of dengue 2 virus 43 strain NS1 gene in transfected BHK-21 cell. Methods:The D2-43 DNA fragment coding for signal peptide plus NS1 protein was cloned between KpnⅠ site and EcoR Ⅰ site of expression plamid pcDNA3.1. The obtained recombinant vector pcDNA-NS1 was transfected into BHK-21 cells with electroporation technique. After selection by G418, resistant clones were screened by RT-PCR and Western blotting test. Results:The RT-PCR results of four in five randomly selected cell clones were positive. Western blotting test showed that NS1 gene could be expressed in BHK-21 cells. Conclusions:NS1 protein was capable of being expressed and appropriately processed in pcDNA-NS1 transfected BHK-21 cells. The present results suggest the feasibility of NS1-based DNA immunization.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Bulletin of The Academy of Military Medical Sciences
Ano de publicação:
2001
Tipo de documento:
Artigo
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