Comparion of the expression of CS3 fimbriae in different vector systems / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
;
(6): 1-4, 2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-642695
ABSTRACT
Objective:
To choose the best vector for the expression of CS3 fimbriae.Methods:
The CS3 operon was cloned into different plasmid vectors such as pUC19 and pTrc99A. The expression of CS3 was monitored by whole-cell ELISA and SDS-PAGE analysis. The assembly of CS3 fimbriae was detected with electron microscopy.Results:
The expression level of CS3 fimbriae using plasmid pUC19 as carrying vector was the highest, and the insertion orientation of CS3 gene into the plasmid has a little effect on its expression level. The expression of CS3 fimbriae was confirmed by SDS-PAGE analysis and electron microscopy.Conclusions:
The promotor of CS3 itself played the key role in the expression of CS3 fimbriae and the copy number of plasmid was the main factor to affect the expression level.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Bulletin of The Academy of Military Medical Sciences
Ano de publicação:
2001
Tipo de documento:
Artigo
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