Comparison of Fungus Detection between PCR and Fungus Culture in Chronic Sinusitis and Normal Controls / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 666-670, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-643833
ABSTRACT
BACKGROUND AND OBJECTIVES:
Fungi are found in normal flora in various amounts. However, fungi in chronic sinusitis are poorly understood, and it is not clearly defined whether it is a pathogen or simply a part of normal flora. The polymerase chain reaction (PCR) is proposed as a more reliable test to detect fungal DNA than the conventional culture technique. The aim of this study was to compare the sensitivity of conventional culture technique and PCR analysis in detecting fungus of the nasal cavity and to compare fungal species found in normal controls and patients with chronic sinusitis. MATERIALS ANDMETHOD:
Sterile saline irrigation was performed and then mucin filled saline was collected from the nasal cavity of patients with chronic sinusitis and normal controls. Fungus culture and PCR analysis were carried out with irrigated saline.RESULTS:
PCR for panfungal gene was positive in 92.5% and 97.5% while fungus cultures were positive in 23.3% and 30.5% of patients with chronic sinusitis and normal controls, respectively. There was no significant difference in the prevalence of fungus and species of fungus between patients with chronic sinusitis and normal controls.CONCLUSION:
PCR for panfungal gene was more sensitive for fungus detection than fungus culture in patients with chronic sinusitis and normal controls. Presence of fungus alone was insufficient to implicate it as the pathogen of chronic sinusitis. It will be necessary to study further about fungal species in patients with chronic sinusitis and normal controls.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sinusite
/
DNA Fúngico
/
Reação em Cadeia da Polimerase
/
Prevalência
/
Técnicas de Cultura
/
Fungos
/
Mucinas
/
Cavidade Nasal
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Otolaryngology - Head and Neck Surgery
Ano de publicação:
2002
Tipo de documento:
Artigo
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