Effect of low-level laser therapy on bisphosphonate-treated osteoblasts / 대한악안면성형재건외과학회지
Maxillofacial Plastic and Reconstructive Surgery
;
: 48-2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-64404
ABSTRACT
BACKGROUND:
This study investigates the effect of alendronate-treated osteoblasts, as well as the effect of low-level laser therapy (LLLT) on the alendronate-treated osteoblasts. Bisphosphonate decreases the osteoblastic activity. Various treatment modalities are used to enhance the bisphosphonate-treated osteoblasts; however, there were no cell culture studies conducted using a low-level laser.METHODS:
Human fetal osteoblastic (hFOB 1.19) cells were treated with 50 μM alendronate. Then, they were irradiated with a 1.2 J/cm² low-level Ga-Al-As laser (λ = 808 ± 3 nm, 80 mW, and 80 mA; spot size, 1 cm²; NDLux, Seoul, Korea). The cell survivability was measured with the MTT assay. The three cytokines of osteoblasts, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) were analyzed.RESULTS:
In the cells treated with alendronate at concentrations of 50 μM and higher, cell survivability significantly decreased after 48 h (p < 0.05). After the applications of low-level laser on alendronate-treated cells, cell survivability significantly increased at 72 h (p < 0.05). The expressions of OPG, RANKL, and M-CSF have decreased via the alendronate. The RANKL and M-CSF expressions have increased, but the OPG was not significantly affected by the LLLT.CONCLUSIONS:
The LLLT does not affect the OPG expression in the hFOB cell line, but it may increase the RANKL and M-CSF expressions, thereby resulting in positive effects on osteoclastogenesis and bone remodeling.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Linhagem Celular
/
Citocinas
/
Fator Estimulador de Colônias de Macrófagos
/
Remodelação Óssea
/
Técnicas de Cultura de Células
/
Alendronato
/
Terapia com Luz de Baixa Intensidade
/
Osteoprotegerina
/
Seul
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Maxillofacial Plastic and Reconstructive Surgery
Ano de publicação:
2016
Tipo de documento:
Artigo
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