In Vitro Antiviral Activity of Sakuranetin against Human Rhinovirus 3
Osong Public Health and Research Perspectives
;
(6): 415-420, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-644179
ABSTRACT
OBJECTIVES:
Rhinoviruses (RVs) cause common cold and are associated with exacerbation of chronic inflammatory respiratory diseases. Until now, no clinically effective antiviral chemotherapeutic agents to treat diseases caused by human rhinoviruses (HRVs) have been reported. We assessed the anti-HRV3 activity of sakuranetin isolated from Sorbus commixta Hedl. in human epithelioid carcinoma cervix (HeLa) cells, to evaluate its anti-rhinoviral potential in the clinical setting.METHODS:
Antiviral activity and cytotoxicity as well as the effect of sakuranetin on HRV3-induced cytopathic effects (CPEs) were evaluated using the sulforhodamine B (SRB) method using CPE reduction. The morphology of HRV3-infected cells was studied using a light microscope.RESULTS:
Sakuranetin actively inhibited HRV3 replication and exhibited antiviral activity of more than 67% without cytotoxicity in HeLa cells, at 100 μg/mL. Ribavirin showed anti-HRV3 activity similar to that of sakuranetin. Treatment of HRV-infected HeLa cells with sakuranetin visibly reduced CPEs.CONCLUSION:
The inhibition of HRV production by sakuranetin is mainly due to its general antioxidant activity through inhibition of viral adsorption. Therefore, the antiviral activity of sakuranetin should be further investigated to elucidate its mode of action and prevent HRV3-mediated diseases in pathological conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rhinovirus
/
Ribavirina
/
Técnicas In Vitro
/
Células HeLa
/
Colo do Útero
/
Resfriado Comum
/
Adsorção
/
Sorbus
/
Métodos
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Osong Public Health and Research Perspectives
Ano de publicação:
2017
Tipo de documento:
Artigo
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