Expression of Senescence-related Proteins in Rat Fracture Callus / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 479-484, 2001.
Article
em Ko
| WPRIM
| ID: wpr-646400
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To evaluate the expression of senescence-related proteins according to the aging process and to determine the role of senescence-related proteins in the bone tissue and their effects on the process of bone union. MATERIALS AND METHODS: 18 Sprague-Dawley rats (8 weeks old: 7, 32 weeks old: 6, and 70 weeks old: 5) were used in the experiment. A unilateral closed femur fracture was made, and the fracture callus was obtained 2 weeks after the fracture. The ossification process was observed in proliferative chondrocytes, the hypertrophic chondrocytes, and in the mesenchymal layer individually by immunohistochemistry, using p16, p21, c-fos and c-jun antibodies. RESULTS: There was no significant differences in the manifestation of p-16, p-21, c-fos, c-jun gene according to the age. The positive ratio of p-16 was maximal in proliferative chondrocytes (54.93%) and decreased in the mesenchymal layer (46.48%), and in hypertrophic chondrocytes (10.85%), in order. The positive ratio of c-fos was maximal in proliferative chondrocytes (73.32%) and decreased in the mesenchymal layer (51.84%), and in hypertrophic chondrocytes (9.64%), in order. CONCLUSION: We believe that senescent genes in the bone tissue participate in the differentiation of osteochondral cells and in the process of fracture callus ossification.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Osso e Ossos
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Calo Ósseo
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Envelhecimento
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Imuno-Histoquímica
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Genes jun
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Ratos Sprague-Dawley
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Condrócitos
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Fêmur
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Anticorpos
Limite:
Animals
Idioma:
Ko
Revista:
The Journal of the Korean Orthopaedic Association
Ano de publicação:
2001
Tipo de documento:
Article