Comparison of the Effects of NOS Inhibitor or HS-1580 on Nigrostriatal Dopaminergic System in MPTP-induced Parkinson's Disease Animal Model / 대한해부학회지
Korean Journal of Anatomy
; : 285-293, 2005.
Article
em Ko
| WPRIM
| ID: wpr-646452
Biblioteca responsável:
WPRO
ABSTRACT
The antioxidant effects of HS-1580 derived from Brown seaweeds in comparison with NOS inhibitor, L-NAME, were tested in the Parkinson's disease animal model. C57BL/6 mice were implanted with osmotic pump containing vehicle, HS-1580 or L-NAME intraperitoneally 2 days before MPTP injection. The Parkinson's animal model were prepared by intraperitonal injection of MPTP (20 mg/kg, 4 times with 2 hours intervals in a ay).The mice were perfused with Zamboni fixative 2 days or 7days after MPTP injection. The 30 micrometer cryostat section were immunostained with free-floating method. Rabbit anti-tyrosine hydroxylase (TH)and rat anti-mouse MAC-1 were used as the primary antibodies. The following results were obtained. The number of TH-immunoreactive (ir)neurons in substantia nigra (SN)and the relative density of TH-ir axon terminals in striatum were decreased by MPTP injection. But the ecrease was significantly attenuated with 10%HS-1580 or L-NAME (50 mg/kg)pretreatment before MPTP injection. MAC-1-ir activated microglia were observed in substantia nigra 2 days after MPTP injection. Activated microglia were showed as thickened processes with round cell bodies. The morphological changes of MAC-1-ir activated microglia were inhibited by HS-15980 or L-NAME pretreatment before MPTP injction. Above results mean that the damage of nigrostriatal dopaminergic system was rescued by pretreatment of HS-1580 or L-NAME in MPTP-induced Parkinson's disease animal model.
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Índice:
WPRIM
Assunto principal:
Doença de Parkinson
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Gravidade Específica
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Substância Negra
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1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina
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Terminações Pré-Sinápticas
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Microglia
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NG-Nitroarginina Metil Éster
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Modelos Animais
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Anticorpos
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Antioxidantes
Limite:
Animals
Idioma:
Ko
Revista:
Korean Journal of Anatomy
Ano de publicação:
2005
Tipo de documento:
Article