Deoxycholic Acid-Induced Signal Transduction in HT-29 Cells: Role of NF-kappa B and Interleukin-8 / 대한소화기학회지
The Korean Journal of Gastroenterology
;
: 176-185, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-64704
ABSTRACT
BACKGROUND/AIMS:
Deoxycholic acid (DCA) has been appeared to be an endogenous colon tumor promoter. In this study, we investigated whether DCA induces nuclear factor-kappa B (NF-kappa B) activation and IL-8 expression, and tauroursodeoxycholic acid (TUDC) inhibits this signaling in HT-29 cells.METHODS:
After DCA treatments, time courses of NF-kappa B binding activity were determined by electrophoretic mobility shift assay (EMSA). Also, we performed Western blotting of I kappa B alpha to confirm NF-kappa B activation. Time and concentration courses of DCA-induced secretion of IL-8 were measured with ELISA in supernatants of cultured media from the cells. To evaluate the role of NF-kappa B, IL-8 levels were assessed after pretreatment with using phosphorothioate-modified anti-sense oligonucleotides (ODN). Moreover, DCA-induced secretions of IL-8 were measured after pretreatment with TUDC.RESULTS:
DCA dose-dependently induced prominent NF-kappa B binding complexes from 30 min to 8 hr and degradation of I kappa B alpha. The secretions of IL-8 were increased with DCA (50~200 micro M) treatment in a time and dose-dependent manner. Pre-incubation of the cells with TUDC (0.1~10 micro M) for 2 hours caused significant decreases in DCA induced IL-8 secretion. However, transient transfection using p50 or p65 AS-ODN showed no effect on IL-8 secretion.CONCLUSIONS:
DCA may play as a colonic tumor promoter through anti-apoptotic effect of NF-kappa B activation and IL-8 expression, and DCA-induced NF-kappa B independent IL-8 expression is inhibited by TUDC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácido Tauroquenodesoxicólico
/
Resumo em Inglês
/
Transdução de Sinais
/
Ativação Transcricional
/
Western Blotting
/
Interleucina-8
/
NF-kappa B
/
Oligonucleotídeos Antissenso
/
Neoplasias do Colo
/
Células HT29
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Gastroenterology
Ano de publicação:
2004
Tipo de documento:
Artigo
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