Role of IL-1alpha in Cisplatin-Induced Acute Renal Failure in Mice
The Korean Journal of Internal Medicine
;
: 187-194, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-64775
ABSTRACT
BACKGROUND/AIMS:
For unknown reasons, caspase-1 -/- mice, protected against cisplatin-induced acute renal failure (ARF), are deficient in interleukin (IL)-1alpha. We thus asked whether IL-1alpha deficiency underlies the mechanism of protection against cisplatin-induced ARF in these mice.METHODS:
Cisplatin (30 mg/kg) was injected intraperitoneally into wild-type C57BL/6 mice to produce a cisplatin-induced model of ARF. IL-1alpha was measured in control vehicle- and cisplatin-treated wild-type animals. We also examined whether IL-1alpha -/- mice were similarly protected against cisplatin-induced ARF. Additionally, infiltration of CD11b- and CD49b-positive cells, as markers of macrophages, natural killer, and natural killer T cells (pan-NK cells), was investigated in wild-type and IL-1alpha -/- mice.RESULTS:
Compared with vehicle-treated mice, renal IL-1alpha increased in cisplatin-treated wild-type mice beginning on day 1. IL-1alpha -/- mice were shown to be protected against cisplatin-induced ARF. No significant difference in the infiltration of neutrophils or CD11b- and CD49b-positive cells were observed between wild-type and IL-1alpha -/- mice.CONCLUSIONS:
Mice deficient in IL-1alpha are protected against cisplatin-induced ARF. The lack of IL-1alpha may explain, at least in part, the protection against cisplatin-induced ARF observed in caspase-1 -/- mice. Investigation of the protective mechanism (s) in IL-1alpha -/- mice in cisplatin-induced ARF merits further study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Tempo
/
Nitrogênio da Ureia Sanguínea
/
Camundongos Transgênicos
/
Células Matadoras Naturais
/
Biomarcadores
/
Imunofluorescência
/
Cisplatino
/
Apoptose
/
Creatinina
/
Infiltração de Neutrófilos
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Internal Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
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