Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering
Tissue Engineering and Regenerative Medicine
;
(6): 755-762, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-648011
ABSTRACT
To develop decellularized heart valve scaffold from porcine for heart valve regeneration. Porcine heart valves were decellularized with unique optimized approach by using 1% sodium dodecyl sulfate solution and 5% dimethyl sulfoxide for the first time. Effect of decellularization process on scaffold were characterized by hematoxylin-eosin, 4′,6-diamidino-2-phenylindole, Masson's trichrome, alcian blue staining and scanning electron microscopy for extracellular matrix (ECM) analysis in scaffold. The results showed that developed protocol for decellularization of heart valve scaffold shown complete removal of all cellular components, without changing the properties of ECM. The developed protocol was successfully used for heart valve ECM scaffolds development from porcine. The developed protocol seems to be promising solution for the heart valve tissue engineering application.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Regeneração
/
Dodecilsulfato de Sódio
/
Microscopia Eletrônica de Varredura
/
Dimetil Sulfóxido
/
Engenharia Tecidual
/
Detergentes
/
Azul Alciano
/
Matriz Extracelular
/
Coração
/
Valvas Cardíacas
Idioma:
Inglês
Revista:
Tissue Engineering and Regenerative Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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