Inhibition of Allergic Response by CpG Motif Immunostimulatory Oligodeoxynucleotide in a Murine Model of Allergic Rhinitis / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: The classic immunotherapy has the inconvenience and the risk of anaphylaxis. Recent reports revealed that immunostimulatory DNA sequences (CpG motif, ISS-ODN) have been shown to act as strong Th1 response-inducing adjuvants and DNA based vaccination might be an effective therapeutic option for the treatment of allergic disease. In our study, we investigated whether ISS-ODN have anti-allergic effect in the mouse rhinitis model of Dermatophagoides farinae (Der f), the most common allergen in Korea. MATERIALS AND METHOD: C57BL/6 mice were sensitized with crude extract of Der f extract. After injection of ISS-ODN, several parameters of allergic response were evaluated. RESULTS: Injection with ISS-ODN suppressed itching symptom, eosinophilic infiltration into nasal mucosa and the splenic T cell proliferation after Der f allergen challenge in the murine model of allergic rhinitis. However, injection with ISS-ODN did not show a detectable Der f-specific IgE suppression. CONCLUSION: Our findings suggested ISS-ODN have an anti-allergic effect in the allergic rhinitis model of Der f allergen. House dust mite allergy can be reduced by the injection with ISS-ODN.