Stimulus-dependent Differential Regulation of JNK1 and JNK3 by JIP1 Scaffold Protein in Human Neuroblastoma, SH-SY5Y / 대한해부학회지
Korean Journal of Anatomy
; : 47-53, 2006.
Article
em En
| WPRIM
| ID: wpr-651963
Biblioteca responsável:
WPRO
ABSTRACT
Activation of c-Jun N-terminal kinase (JNK) is associated with a wide range of disparate cellular responses to extracellular stimuli. In mammals, three JNK isoforms are known, and their differential regulation occurs in a stimulus- or a cell type-dependent manner. However, the underlying mechanism of this differential regulation has not been clearly elucidated. Here we demonstrated that JNK1 and JNK3 were activated in SH-SY5Y cells after treatment with H2O2 or UV. In SH-SY5Y cells overexpressing mJIP1, a splicing variant of a JNK scaffold protein JIP1, the H2O2-induced activities of both JNK1 and JNK3 were significantly suppressed. In the same cell line, however, UV-induced JNK1 activity was significantly suppressed, but JNK3 activity was not. During the RA-induced differentiation of SH-SY5Y cells, JNK1 was activated, whereas JNK3 was not, and this JNK1 activation was completely abolished in the cells overexpressing mJIP1. These results suggest that JIP1 plays a role in the regulation of the isoform-specific activation of JNKs in stimulus-dependent manner.
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Índice:
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Assunto principal:
Linhagem Celular
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Isoformas de Proteínas
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Proteínas Quinases JNK Ativadas por Mitógeno
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Mamíferos
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Neuroblastoma
Limite:
Humans
Idioma:
En
Revista:
Korean Journal of Anatomy
Ano de publicação:
2006
Tipo de documento:
Article