Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces / 대한수의학회지
Korean Journal of Veterinary Research
;
: 55-57, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-65249
ABSTRACT
Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) has extended latent periods of infection. Due to this property, difficulties in the detection of fecal shedder have been raised. A newly designed method for DNA extraction from fecal specimens, mGITC/SC was evaluated in terms of diagnostic efficiency. The detection limit of IS900 real-time PCR was about 50 MAP (1.5 cfu) in 250 mg of feces (6 cfu per g). Also, this DNA extraction method was faster and cheaper than that using commercial kit or other methods. Consequently, the mGITC/SC is an economical DNA extraction method that could be a useful tool for detecting MAP from fecal specimens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Paratuberculose
/
DNA
/
Fezes
/
Limite de Detecção
/
Reação em Cadeia da Polimerase em Tempo Real
/
Mycobacterium
/
Mycobacterium avium
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS