Matrix Metalloproteinase(MMP)-2 Expression Related to Stretch Injury of the Anterior Cruciate Ligament in the Knee / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 643-649, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-652866
ABSTRACT
PURPOSE:
Tissue remodeling is essential for the natural healing of damaged tissue. The matrix metalloproteinases (MMPs) that degrade the extracellular matrix to play a central role in this process. This study was designed to examine the basic relationship between MMP-2 and anterior cruciate ligament (ACL) injury. MATERIALS ANDMETHODS:
After the femur-ACL-tibia complex was harvested from rats, the continuous release of MMP-2 in the stretch group was evaluated. The complexes were divided into three groups. To stretch the complexes, in group I, 5 and 12 N weights were used for 10, 30, and 60 minutes. In group II, 5 and 15 N weights were used for 5, 10, 30, 60, and 120 minutes. And in group III, 5, 8, 10, 12, and 15 N weights were used for 30 and 60 minutes. MMP-2 activity was evaluated using 0.05% gelatin zymography.RESULTS:
MMP-2 was expressed and the signal intensity was increased according to the stretch duration and magnitude. In the tissue analysis, active MMP-2 was markedly present and the expression was increased in response to the stretch in the supernatant samples.CONCLUSION:
MMP-2 was released immediately after ligament injury and its activity was increased in proportion to the stretch duration and magnitude. It is believed that MMP-2 expression plays an important role in the remodeling process after an ACL injury.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pesos e Medidas
/
Ligamento Cruzado Anterior
/
Metaloproteinases da Matriz
/
Matriz Extracelular
/
Gelatina
/
Joelho
/
Ligamentos
Limite:
Animais
Idioma:
Coreano
Revista:
The Journal of the Korean Orthopaedic Association
Ano de publicação:
2006
Tipo de documento:
Artigo
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