Osteogenic Differentiation of Human Mesenchymal Stem Cells: Effects of the compositions of biodegradable polymers and the initial cell-seeding density / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 259-266, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-652941
ABSTRACT
PURPOSE:
To investigate the effects of initial cell-seeding density and the compositions of biodegradable polymer scaffolds on the osteogenic differentiation of human mesenchymal stem cells. MATERIALS ANDMETHODS:
Human mesenchymal stem cells (hBMSCs) were cultured three-dimensionally onto biodegradable polymers with three different compositions (65/35 PLGA, 85/15 PLGA, PLLA), and osteogenic differentiation was studied by; histology, immuno-histochemistry, and transmision/scanning electron microscopy.RESULTS:
In terms of the amount of extracellular matrix (ECM) produced, the 65/35 PLGA (23% of the whole volume) was found to produce more ECM than 85/15 PLGA (21%) and PLLA (13%). Osteoblastic differentiation of hBMSCs was induced in all scaffolds examined and the deposition of ECM with calcifications started after 2 weeks of culture. The amount of calcium deposited on the 65/35 PLGA dramatically increased at 4 weeks. On comparing low cell-seeding density with the high cell-seeding density on 65/35 PLGA, calcium deposits reached similar levels at 6 weeks.CONCLUSION:
Three-dimensional culture of hBMSCs on biodegrable polymer scaffolds induced bone formation, which was dependent on the compositions of the scaffolds and the initial cell-seeding density.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Osteogênese
/
Polímeros
/
Microscopia Eletrônica
/
Cálcio
/
Matriz Extracelular
/
Células-Tronco Mesenquimais
Limite:
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Orthopaedic Association
Ano de publicação:
2003
Tipo de documento:
Artigo
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