Myoblast Transfer Therapy on mdxMouse / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 281-287, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-653267
ABSTRACT
PURPOSE:
To observe dystrophin formation and histological improvement in dystrophic muscle of mdx mouse after normal myoblast injection. MATERIALS ANDMETHODS:
Cultured myoblasts from genetically normal rats were injected into the right quadriceps femoris of a 6-week-old mdx mouse (n=9). dPBS was injected into the left quadriceps femoris as a control. One, 2, and 3 months after injection, The control and experimental group were compared histologically and by dystrophin immunostaining.RESULTS:
When compared with controls 3 months postoperatively, quadriceps femoris in the experimental group exhibited greater cross-sectional area and total fiber number, and the experimental animals contained more normal-appearing and less abnormalappearing fibers than the control group. Most of the fibers in the experimental group showed positive results in dystrophin immunostaining, whereas immunostaining of mdx muscle fibers in the control group was completely negative.CONCLUSION:
This study shows that normal myoblast injection improved the muscle architecture histologically and produced dystrophin protein in dystrophic muscle.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Distrofina
/
Camundongos Endogâmicos mdx
/
Mioblastos
/
Músculo Quadríceps
/
Distrofias Musculares
Limite:
Animais
Idioma:
Coreano
Revista:
The Journal of the Korean Orthopaedic Association
Ano de publicação:
2002
Tipo de documento:
Artigo
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