The Effect of Tumor Necrosis Factor-Alpha to Glomerular Epithelial Cells in Glomerular Permeability / 대한해부학회지
Korean Journal of Anatomy
;
: 389-395, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-654601
ABSTRACT
Minimal Change Disease (MCD) is the most common primary nephrotic syndrome in children. Some suggested that tumor necrosis factor-alpha (TNF-alpha) are involved in the pathogenesis of MCD. This study was done to see changes of plasma and urinary TNF-alpha, and its effect on determination of permeability of glomerular basement membrane (BM) contributed by heparan sulfate proteoglycan (HSPG). Study patients consisted of 19 biopsy-proven MCD children aged 2-15 years old. Both plasma and urinary TNF-alpha were measured. Employing the Millicell system, TNF-alpha was screened for the permeability factors. We examined whether TNF-alpha regulated BM HSPG gene expression and HS synthesis in the glomerular epithelial cells (GECs). Urinary TNF-alpha during relapse was also significantly increased (364.4+/-51.2 vs 155.3+/-20.8, 36.0+/-4.5 ng/mg.cr) (p<0.05). However, the negative results were obtained in the permeability assay using the Millicell system. No difference was seen in BM HSPG gene expression and HS synthesis in the GECs. Therefore, it seems that TNF-alpha may not play a disease-specific role in the pathogenesis of MCD.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Permeabilidade
/
Plasma
/
Recidiva
/
Expressão Gênica
/
Fator de Necrose Tumoral alfa
/
Proteoglicanas de Heparan Sulfato
/
Células Epiteliais
/
Membrana Basal Glomerular
/
Nefrose Lipoide
/
Síndrome Nefrótica
Limite:
Criança
/
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Anatomy
Ano de publicação:
2003
Tipo de documento:
Artigo
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