The Efficient Gene Delivery into Human Mesenchymal Stem Cells Using Retroviral Vectors / 대한해부학회지
Korean Journal of Anatomy
;
: 381-387, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-654602
ABSTRACT
Human mesenchymal stem cells (hMSCs) are multipotent stem cells that can differentiate into several mesenchymal lineage cells. In this study, we established an efficient method for gene delivery into these cells. Non-viral transfection reagents that were commercially available yielded 5% efficiency. In contrast, a retroviral vector yielded more than 46% transduction, which was further increased to 90% by repetitive infection. Retroviral transduction did not alter the multipotency of hMSCs. Thus, the cells retained the potential to differentiate into adipogenic, chondrogenic, or osteogenic lineages. The conditions established in this study will contribute to development of trans-differentiation methods of hMSCs into non-mesodermal lineage cells and thereby facilitate their possible use as vehicles for autologous transplantation in both cell and gene therapy for various diseases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Retroviridae
/
Transplante Autólogo
/
Transfecção
/
Terapia Genética
/
Zidovudina
/
Células-Tronco Multipotentes
/
Células-Tronco Mesenquimais
/
Autoenxertos
/
Indicadores e Reagentes
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Anatomy
Ano de publicação:
2003
Tipo de documento:
Artigo
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