Screening of Low Molecular Metabolite, FS390 as an Inhibitor of Neurotransmitter Release from PC12 Cells / 대한해부학회지
Korean Journal of Anatomy
;
: 91-102, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-656242
ABSTRACT
We established an in vitro experimental system using the following procedure. We first introduced tritium-labeled norepinephrine ([3H]-NE) into PC12 cells. The [3H]-NE incorporated-PC12 cells were stimulated by a high concentration (60 mM) of K+ buffer during 12 minutes. Then, we collected 100 microliter supernatant and counted the amount of [3H]-NE release from PC12 cells with a scintillation counter. After screening fungal, Streptomyces spp. or bacterial product using this experimental sytem, we obtained FS390 from Streptomyces spp. which inhibited [3H]-NE release from PC12 cells. FS390 also inhibits the release of ATP as a neurotransmitter of PC12 cells and rat cortical neurons. The inhibitory effect was seen even when the PC12 cells were treated with low K+ buffer containing ionomycin (1 micrometer) as an ionopore. This result suggests that the inhibitory action of FS390 on neurotransmitter release appeared after the influx of Ca2+.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Contagem de Cintilação
/
Streptomyces
/
Norepinefrina
/
Ionomicina
/
Trifosfato de Adenosina
/
Programas de Rastreamento
/
Células PC12
/
Neurotransmissores
/
Exocitose
/
Neurônios
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Anatomy
Ano de publicação:
2006
Tipo de documento:
Artigo
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