Screening of Low Molecular Metabolite, FS390 as an Inhibitor of Neurotransmitter Release from PC12 Cells / 대한해부학회지
Korean Journal of Anatomy
; : 91-102, 2006.
Article
em Ko
| WPRIM
| ID: wpr-656242
Biblioteca responsável:
WPRO
ABSTRACT
We established an in vitro experimental system using the following procedure. We first introduced tritium-labeled norepinephrine ([3H]-NE) into PC12 cells. The [3H]-NE incorporated-PC12 cells were stimulated by a high concentration (60 mM) of K+ buffer during 12 minutes. Then, we collected 100 microliter supernatant and counted the amount of [3H]-NE release from PC12 cells with a scintillation counter. After screening fungal, Streptomyces spp. or bacterial product using this experimental sytem, we obtained FS390 from Streptomyces spp. which inhibited [3H]-NE release from PC12 cells. FS390 also inhibits the release of ATP as a neurotransmitter of PC12 cells and rat cortical neurons. The inhibitory effect was seen even when the PC12 cells were treated with low K+ buffer containing ionomycin (1 micrometer) as an ionopore. This result suggests that the inhibitory action of FS390 on neurotransmitter release appeared after the influx of Ca2+.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Contagem de Cintilação
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Streptomyces
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Norepinefrina
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Ionomicina
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Trifosfato de Adenosina
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Programas de Rastreamento
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Células PC12
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Neurotransmissores
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Exocitose
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Neurônios
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
Ko
Revista:
Korean Journal of Anatomy
Ano de publicação:
2006
Tipo de documento:
Article