Detection of Specific lgE Antibody from Nasal Secretion in Allergic Rhinitis with Negative Results of Skin Test and RAST on D.P / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: The diagnosis of allergic rhinitis due to specific antibody is essentially dependent upon the patients' clinical histories, physical examinations, skin test results, and especially on the immunological technique in vitro used for the detection for specific IgE antibody in serum. However, some patients with positive results from nasal provocation tests with allergen can have the negative results from skin tests and the immunological technique in vitro. Therefore, systemic allergic reactions are not completely correlative to those of the localized allergy. MATERIALS AND METHODS: This study was therefore designed to explore the relationship between RAST (radioallergosorbent test) results in nasal secretion of 6 localized allergic patients and 10 controls (5 systemic allergic and 5 non-allergic patients). The nasal secretion was collected by nasal suction method each in three groups before and 20 minutes after antigen (Dermatophagoides pteronyssinus) nasal spray, respectively. RESULTS: There were negative RAST results in nasal secretion of localized allergic patients and controls (5 non- allergy patients) before the antigen nasal spray but, positive RAST results in those of localized allergic patients and controls (5 systemic allergic patients) after the antigen nasal spray. This means that there was statistically a significant difference between localized allergic patients and controls of non-allergy patients after the antigen nasal spray (p<0.01). CONCLUSION: We suggest that localized al1ergic reaction is a specific antigen-antibody allergic immunologic reaction and RAST in nasal secretion is good assistant diagnostic technique in localized allergy.