Your browser doesn't support javascript.
loading
Effect of Ginseng Saponin on Cultured Osteoblast / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association ; : 868-876, 1998.
Artigo em Coreano | WPRIM | ID: wpr-656732
ABSTRACT
Earlier work suggested that Ginseng saponin had an effect on healing of fracture and osteoporosis, hut there has heen no report on saponin effects on cultured osteoblast. The purpose of this study was to investigate the effect of saponin on cultured osteovlast of the mouse. Osteohlastic cells were isolated from periosteum-stripped parietal bones of neonatal rat calvaria, seeded at 4 x 10 4 cells/cm2 density, and cultured in standard medium and deficient medium, with addition of saponin (0(control), 10, 20, 50 microgram/ml concentration). Plating efficiency(adhesiveness), proliferation and the alkaline phosphatase activity of the osteohlasts were evaluated on 2 hours, days 2 and 7 of culture. Saponin was found to enhance the adhesiveness of seeded osteoblasts which were cultured with a deficient medium in comparison to the controls. It promoted cell proliferation of the osteoblasts cultured with a deficient medium in comparison to the controls. There was also a significantly enhanced increase of alkaline phosphatase levels in the presence of saponin during culture with deficient medium in comparison to the controls. In summary, the results showed that the saponin increased adhesiveness, proliferation and the alkaline phosphatase activity of the osteoblast cultured only in deficient medium.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Osteoporose / Osso Parietal / Saponinas / Crânio / Adesividade / Proliferação de Células / Fosfatase Alcalina / Panax Limite: Animais Idioma: Coreano Revista: The Journal of the Korean Orthopaedic Association Ano de publicação: 1998 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Osteoporose / Osso Parietal / Saponinas / Crânio / Adesividade / Proliferação de Células / Fosfatase Alcalina / Panax Limite: Animais Idioma: Coreano Revista: The Journal of the Korean Orthopaedic Association Ano de publicação: 1998 Tipo de documento: Artigo