Mechanism of salvianolate regulating activity of NLR family pyrin domain containing 3 inflammasome in treatment of mice with colitis / 中华消化杂志

ABSTRACT

Objective To study the role of salvianolate in the treatment of inflammation of intestinal mucosa by colitis mice model.Methods A total of 60 C57BL/6J mice were divided into acute control group,acute model group,acute interventional group,chronic control group,chronic model group and chronic interventional group with 10 mice in each group.Dextran sodium sulfate (DSS) was used to establish the model.Meanwhile,the mice of interventional group received salvianolate peritoneal injection during modeling.The expression levels of NLR family pyrin domain containing 3 (NLRP3),apoptosis-assoeiated speck-like protein containing CARD (ACS) and cysteinyl aspartate specific proteinase 1 (caspase-1) were detected.And the absorbances of serum superoxide dismutase (SOD) and malonaldehyde (MDA) in mice serum were determined.Kruskal Wallis single factor analysis of variance,single factor analysis of variance,Student-Newman-Keuls (SNK) method and least-significant difference method were performed for statistical analysis.Results The expressions of NLRP3 at protein level of acute control group,acute model group,acute interventional group,chronic control group,chronic model group and chronic interventional group were 9 965.20 (196.81),16 703.38 (291.21),13 423.74 (209.28),10 112.01 (183.55),16 247.90 (505.44) and 12 674.95 (229.32),respectively.The expressions of NLRP3 of acute and chronic interventional group were both lower than those of acute and chronic model group,and the differences were statistically significant (both x2 =15.158,P＜0.01).The expressions of ACS at protein level were 9 744.09-±-546.58,16 555.44 ± 407.80,12 568.21±586.49,10066.32±435.63,14 911.73±384.51 and 13 751.37±322.30,respectively.The expressions of caspase-1 at protein level were 5 300.40±843.14,15 478.98± 174.09,10 587.46±821.60,5 517.28±876.16,13 164.01 ±416.47 and 8 856.27 ± 545.01,respectively.The expressions of ACS and caspase-1 of acute and chronic interventional groups were both lower than those of model groups,and the differences were statistically significant (F=260.56,329.36,240.38 and 236.26,all P＜0.01).The serum levels of SOD were (282.08±28.24),(221.87±5.81),(231.45±1.84),(267.08±43.63),(237.09±34.09) and (257.78 ± 4.68) U/mL,respectively.There was no significant difference between acute model group and acute interventional group (P＞0.05).SOD level of chronic interventional group was higher than that of model group,and the difference was statistically significant (t=-2.65,P =0.021).The serum levels of MDAwere (1.31±0.40),(6.95±1.57),(2.98±1.41),(1.21±0.16),(9.38±3.73)and (3.74±0.81) nmol/L,respectively.MDA levels of acute and chronic interventional groups were lower than those of acute and chronic model groups,and the differences were statistically significant (t=4.88 and 4.02,both P＜0.01).Conclusion Salvianolate may alleviate oxidative stress response by adjusting the activity of NLRP3 inflammasome which takes part in reducing intestinal inflammation.