Changes of IL-17and HCLCA1 Induced by RSV Infection and the Inhibition Effect of Rosiglitazone / 医学研究杂志

ABSTRACT

Objective To observe the expressions of peroxisome proliferator activatedγ (PPARγ),IL-17 and calcium activated family member 1 (hCLCA1) mRNA and protein in A549 cell infected with respiratory syncytial virus(RSV),and to explore the effect of rosiglitazone and anti-IL-17 Ab.Methods A549 cells were seeded in 6-well culture plates and randomly divided into six groups.DMSO/RSV group received medium containing 0.02％ DMSO 30min prior to RSV infection.Rosiglitazone/RSV group received rosiglitazone (101μmol/L) 30 min prior to RSV infection.GW9662/rosiglitazone/RSV group was first exposed to GW9662 (10μmol/L)for 30 min,prior to rosiglitazone and RSV infection.Anti-IL-17 Ab (0.15μmol/L) was administered to anti-IL-17 Ab/RSV group 2 hour prior to RSV infection.Supernatants were harvested at 6h,12h and 24h post-RSV infection,retrospectively.The expression of PPARγ,IL-17,hCLCA1 mRNA were measured by real-time quantitative PCR.Protein levels of hCLCA1 and IL-17 were measured by Western blot and ELISA,retrospectively.Results The levels of PPARγ,IL-17and hCLCA1 mRNA and protein were higher in DMSO/RSV group than those in cell control group (P ＜ 0.05) at three time points.The expression of IL-17,hCLCA1 mRNA and protein were lower both in rosiglitazone/RSV group and anti-IL-17 Ab/RSV group than those in DMSO/RSV group at the same time (P ＜0.05).While,both IL-17,hCLCA1 protein and mRNA expression in GW9662/Rosiglitazone/RSV group were similar to that in DMSO/RSV group (P＜ 0.05).Compared with that at 12h and 24h,rosiglitazone and anti-IL-17 Ab had the strongest inhibition effect onthe expression of IL-17 mRNA and protein at 6h (P ＜ 0.05).Conclusion Rosiglitazone can inhibit RSV-induced expression of IL-17,hCLCA1 and may inhibit airway mucus hypersecretion by increasing the activity of PPARγ.