Inhibitory effect of Gallnut on Candida albicans biofilm / 中国生化药物杂志

ABSTRACT

Objective To study the inhibitory effect of Gallnut on the biofilm of Candida albicans. Methods MTT assay was used to determine the inhibitory effect of Gallnut on biofilm formation and mature biofilm. RT-PCR was used to determine the effect of Gallnut on the expressions of ALS1 and CPH1 genes. Results Gallnut has a stronger inhibitory effect on Candida albicans biofilm formation, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) for inhibition of biofilm formation both are 128mg/mL. Gallnut also has a strong inhibitory effect on the mature biofilm of Candida albicans, the minimum inhibitory concentration (MIC) is 256mg/mL. Gallnut could significantly reduce the gene expressions of ALS1 and CPH1. Compared with the control group, 64 mg/mL Gallnut act on Candida albicans after 16h, the gene expression of ALS1 and CPH1 decreased by 79.2% and 82.0% respectively. Conclusion Gallnut has a significant inhibitory effect on the formation and maturation of Candida albicans biofilm. The inhibitory effect of Gallnut on the adherence and invasion of Candida albicans is mainly through inhibiting the expressions of ALS1 and CPH1 genes.