Effects of the umbilical cord stem cells on alleviating the cardiac injury in severe burn rats / 实用医学杂志

ABSTRACT

Objective To study the protective effects of umbilical cord stem cells on cardiac injury in severely burned rats. Methods 30 SD rats were randomly divided into sham group ,burn group and stem cells treatment group. The severely burned head ,neck and back of rat model of 35%TBSAⅢdegree was eatabished in burn and stem cells treatment groups. The blood and cardiac tissues were collected at 24 hours after stem cells transplantation. The distribution patterns of umbilical cord stem cells in cardiac tissues ,and infiltrations of neutro-phils and macrophages in cardiac tissues were detected by immunohistochemistry staining method. The cardiac structures of rats were evaluated by HE staining. The levels of Troponin I ,Creatine Kinase and Creatine Kinase-MB in serum were detected by the blood biochemical automatic analyzer. Results The umbilical cord stem cells migrated into the injured cardiac tissues and mainly concentrated in capillaries of heart. The umbilical cord stem cells significantly decreased neutrophils and macrophages infiltration of the cardiac tissues ,and markedly alleviated structure damages of the cardiac tissues in severely burned rats. Furthermore ,the levels of Troponin I ,Creatine Kinase,Creatine Kinase-MB of burn group were significantly higher than that of sham group,but those of stem cells treatment group were markedly lower than that of burn group (P < 0.05). Conclution The umbilical cord stem cells transplantation could effectively reduce inflammatory cells infiltration of the cardiac tissues,then protected the structure and function of the heart in severely burned rats.