Analysis of HBV-DNA load and liver function in maternal with different HBV infection patterns / 国际检验医学杂志

ABSTRACT

Objective To study the relationship between the infection patterns of hepatitis B positive maternal and the hepatitis B virus (HBV)-DNA load in serum and the HBV-DNA load in breast milk ,the relationship between HBV-DNA load in the breast milk and maternal liver function ,and evaluate the safety of breast feeding .Methods HBsAg and HBeAg were detected by ELISA in 267 cases of hepatitis B positive maternal ,HBV-DNA load in serum and breast milk was detected by fluorescent quantitative PCR ,and alanine aminotransferase (ALT ) was detected by rate method .Results The positive rate of breast milk HBV-DNA in 109 cases of HBsAg and HBeAg double positive was 76 .1% ,the positive rate of breast milk HBV-DNA in 158 cases of HBsAg positive and HBeAg negative women was 3 .2% ,the difference was statistically significant (P<0 .05) .3 groups of women were observed to carry different loads of HBV-DNA in their serum ,HBV-DNA < 500 ,500 -1 .0 × 105 ,> 1 .0 × 105 copies/mL ,respectively ;the difference of HBV-DNA load in breast milk was statistically significant (P<0 .05) ,and there was a positive correlation between HBV-DNA load in serum and HBV-DNA load in breast milk ;there were significant differences in serum ALT between HBV-DNA positive breast milk maternal and HBV-DNA negative maternal(P<0 .05) .Conclusion HBsAg and HBeAg double positive mater-nal and the maternal whose HBV-DNA load >1 .0 × 105 copies/mL in serum are not appropriated to breast-feeding .Women with high HBV-DNA load in breast milk should monitor liver function at the same time ,so as to prevent liver injury .