Advances in tracing techniques of bone marrow mesenchymal stem cells / 中华骨科杂志

ABSTRACT

The establishment and development of tissue engineering has changed the traditional treatment model which re-pair trauma with trauma. It combines seed cells with biomaterials to repair bone, cartilage, muscle, blood vessels and other tissue defects. Bone marrow mesenchymal stem cells (BMMSCs) are seed cells for stem cell regenerative medicine. They have been exten-sively studied because of many advantages, such as multi-directional differentiation, easy-extraction and culture, strong prolifera-tion ability, low immunogenicity, and so on. However, there are some problems in the repair in trauma of BMMSCs, which restrict their application in clinical medical treatment. Therefore, a great deal of basic experimental studies are still needed. In a large number of experiments on BMMSCs, various types of detection methods emerge in an endless stream. In order to monitor its status like migration, proliferation and differentiation in vivo, tracing technology has been using. Tracing, in short, is the tracking of mark-ers. There are three types of labeling methods, including Report gene, Fluorescent dyes and Nano particle, using one or more of the medical imaging technologies, such as optical imaging, magnetic resonance imaging (MRI) and radionuclide imaging (RNI) to ob-serve. Thus, a variety of tracer techniques are formed. Different tracing technologies have their own advantages and disadvantages, which are related to the nature of the selected marker itself and the characteristics of the imaging technique. In order to obtain bet-ter and more accurate experimental results, researchers are constantly improving and developing new tracing technology. In this paper, we classify tracing techniques by different labeling methods, review and prospect the various tracing techniques currently applied to BMMSCs.