Inhibitory effect of SAMHD1 on production of HBV DNA / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1474-1477, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-660076
ABSTRACT
Objective:
To study the expression levels of SAMHD1 in different liver cancer cells and the inhibition of HBV DNA production by SAMHD1.Methods:
SAMHD1 mRNA and protein levels in Jurkat,THP-1,HepG2,HepG2. 2. 15 and Huh7 were detected by Real-time quantitative PCR and Western blot. SAMHD1 expression in HepG2. 2. 15 was increased by transfecting SAMHD1 over ex-pression vectors or was inhibited by transfecting SAMHD1 specific shRNA, and then HBV DNA levels were detected. The effect of different dNTP concentration on HBV DNA production was detected also.Results:
SAMHD1 were highly expressed in liver cancer cells. Decreased SAMHD1 expression could enhance HBV DNA production by 2. 3-2. 8 times,and increased SAMHD1 expression could inhibit HBV DNA levels by 3 times(P<0. 01). Increased dNTP concentration could enhance HBV DNA production and counteract the inhibitory effect of SAMHD1.Conclusion:
SAMHD1 can inhibit the production of HBV DNA in liver cancer cells by decreasing dNTP concentration.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2017
Tipo de documento:
Artigo
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