Study on the relationship between promoter -579G>T of DNMT3B with genetic susceptibility to gastric cancer in Jiangsu Suqian region / 国际检验医学杂志

ABSTRACT

Objective To investigate the association between DNA methyltransferase 3B(DNM T3B) promoter single nucleotide polymorphism(SNP) with susceptibility to gastric cancer (GC) in Han population of Suqian region .Methods A total of 233 pa-tients with gastric cancer were recruited into the study group ,208 healthy persons were recruited in the control group .Genomic DNA was extracted from peripheral blood ,and the frequency distribution of -579G> T locus in DNMT3B promoter region was de-tected by polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) .Results The distribution of TT genotypes compared with GT +GG in all the two groups were significantly different (χ2 =6 .517 ,P<0 .05) .When stratified by age , there was significant difference between TT and GT + GG genotypes at the age range ≥60 years(χ2 = 4 .728 ,P< 0 .05) .When stratified by gender ,there was no significant difference between different gender groups (χ2 = 3 .541 ,P> 0 .05 ;χ2 = 3 .676 ,P>0 .05) .Conclusion The DNMT3B -579G> T is associated with the genetic susceptibility of gastric cancer .It might be a risk fac-tor of gastric cancer .