Breeding and genotyping of a miR-5572transgenic mouse model of sick sinus syndrome / 中国实验动物学报

ABSTRACT

Objective This study aimed to explore the possibility of establishing a model of sick sinus syndrome by using miR-5572 transgenic mice. Methods F1 and F2 miR-5572 transgenic mice were bred and genotyped, and then observed the phenotype levels of miR-5572 transgenic mice by morphology, electrocardiogram record ( ECG ) and the Cav1. 2 and Cav1. 3 expressions levels of mRNA and protein in sinoatrial node tissue of homozygous, heterozygote and wild type mice. Results Compared with the wild type and heterozygous mice,the miR-5572 homozygous mice showed a devel-opment delay and smaller body shape,and had slower average heart rate. The mRNA and protein levels of Cav1. 2 and Cav1. 3 in the sinoatrial node tissues were significantly lower. Conclusions The results of this study indicate that miR-5572 homozygous mice may be an efficient approach to establish the model of sick sinus syndrome