Typing and homology of drug resistance genes of carbapenem-resistant Enterobacteriaceae / 实用医学杂志

ABSTRACT

Objective To investigate the genotype and its homology of carbapenem-resistant Enterobacter (CRE)isolated from hospitalized patients and to provide theoretical basis for clinical treatment. Methods Drug sensitivity of bacteria to antibiotics widely used in clinic was detected with a VITEK-2 COMPACT fully automated microbiological system. Resistance genes including blaKPC,blaNDM-1,blaIMI-1,blaGES,blaSME and blaSHV were detected by polymerase chain reaction(PCR)assay. Results Thirty carbapenem-resistance strains were col-lected,including e.cloacac(17 strains),e. Aerogenes(10 strains),e. Sakazakii(2strains)and e. Cancerogenus (1strain). Positive genes included blaSHV(20.0%),blaNDM-1(16.7%),blaKPC(6.7%),blaGES(3.3%), blaIMI-1 (0) and blaSME (0). Five strains which harbored blaNDM-1 were isolated into 3types. Conclusions The most prevalence resistance genes among the strains are blaSHV ,followed by blaNDM-1 and other resistance genes at difference levels. There is potentially clonal spread of the resistance genes in the same room or in the whole hospital.