Anti-inflammatory effects of Daphniphyllum calycinum extract / 中成药
Chinese Traditional Patent Medicine
;
(12): 1771-1776, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-661414
ABSTRACT
AIM To study the in vitro and in vivo anti-inflammatory effects of extract from Daphniphyllum calycinum Benth and its mechanism of action.METHODS LPS-induced mouse RAW264.7 macrophage and mouse endotoxemia model were used to observe the influences of D.calycinum extract on the levels of NO,TNF-α,IL-1β and IL-10.The toxicity of D.calycinum extract on RAW264.7 macrophage was determined by MTT assay.Fifty female BALB/c mice were administered with D.calycinum extract by gavage.Fourteen days later,the mice were intraperitoneally injected with LPS,the levels of NO,TNF-α,IL-1 β and IL-10 in serum were determined by Griess and ELISA kit.The protein expressions of NO and TNF-α in mouse liver were studied by immunohistochemistry method.RESULTS D.calycinum extract could significantly inhibit the release of NO,TNF-α,IL-1 β and IL-10.Immunohistochemistry results showed that the extract could inhibit the protein expressions of iNOS and TNF-α.CONCLUSION D.calycinum extract has a significant anti-inflammatory effect,and its mechanism may be related to decreasing the release of inflammatory factors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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