Expression of GRIM-19 in adenomyosis and its mechanism for promoting disease progress / 重庆医学

ABSTRACT

Objective To study the expression level of GRIM-19 in endometrial tissue of the patients with adenomyosis and to investigate its molecular mechanism during disease occurrence and progress process.Methods The ectopic and eutopic endometrial tissues were obtained from 30 patients with adenomyosis.Immunohistochemistry and Western blot were performed to detect the expression of GRIM-19,pSTAT3 and VEGF.The endometrial tissueapoptosis was assayed by TUNEL.Immunohistoehemistry with anti-CD34 antibody was performed to detect angiogenesis in endometrial tissue.GRIM-19 small interfering RNA(siRNA) and recombinant GRIM-19 plasmid were constructed and transfected into Ishikawa cells for detecting the change of downstream signal molecules pSTAT3,STAT3 and VEGF.Results The expression level of GRIM-19 was decreased in the ectopic and eutopic endometrial tissues of patients with adenomyosis compared with control group.Apoptosis in ectopic and eutopic endometrial tissues was reduced;the microvessel density and expression levels of pSTAT3 and VEGF were increased.Transfecting Ishikawa cells and downregulating GRIM-19 expression could significantly activate pSTAT3 and VEGF.Conclusion GRIM-19 promotes the occurrence and development of adenomyosis by inhibiting apoptosis and angiogenesis.