Effects of isoflurane inhalation anesthesia on the expressions of BDNF and Bcl-2 in rat hippocampus / 重庆医学

ABSTRACT

Objective To observe the effects of isoflurane (ISO) inhale anesthesia on the expression of brain-derived neurotrophic factor (BDNF) and Bcl-2 of hippocampal in rats.Methods A total of 54 SD rats were divided into 3 groupscontrol group (n=6),O2 group (n=24) and ISO group (n=24).All rats were given 1 week to adapt the environment.Then,rats in the control group were directly sacrificed to collect hippocampi specimens;rats in the O2 group inhaled mixed gas containing 40％ O2 and air for 1 hour;rats in the ISO group anesthetized with 3 ％ ISO and maintained for 1 h with 1.8 ％ ISO after righting reflex disappeared,inhaling 40％ O2 in the whole process of anesthesia.Respiratory rate and percutaneous oxygen saturation (SpO2) of the rats were observed before anesthesia,after induction,30 min after anesthesia,60 min after anesthesia and at the moment of analepsia,respectively.Rats in the O2 group and ISO group were sacrificed to extract hippocampi specimens at 6,12,24 and 72 h after treatment,respectively.The BDNF and Bcl-2 mRNA expression levels in hippocampus of rats were detected using the semi-quantitative reverse transcription PCR (RT-PCR).Results Compared to that before anesthesia,the respiratory rates after induction,30 min after anesthesia and 60 min after anesthesia were decreased (P＜0.05),while no apparent changes was found in the SpO2 (P＞0.05).Compared to the O2 group,the relative expressions of BDNF mRNA in hippocampi of rats in the ISO group were obviously decreased at 12 and 24 h after the treatment (P＜0.05).After anesthesia,the relative expression of BDNF mRNA in rat hippocampus in the ISO group was decreased as time goes on,and the expression levels at 12 and 24 h after the treatment were lower than those in the control group (P＜0.05).Compared to the O2 group and control group,the relative expression levels of Bcl-2 mRNA in hippocampi of the rats in the ISO group were lower at all time points for detection (P＜0.05).Conclnsion ISO can generate transient inhibition effect on expressions of BDNF and Bcl-2 in hippocampus of rat.