Analysis of Small Molecules by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry with Porous Zeolite Supported α-Cyano-4-Hydroxycinnamic Acid as Composite Matrix / 分析化学

ABSTRACT

Zeolite supportedα-cyano-4-hydroxycinnamic acid ( CHCA) as novel composite matrix was applied in matrix assisted laser desorption ionization time-of-flight mass spectrometry ( MALDI-TOF-MS ) analysis of substance P and small molecular compounds of fluoroquinolones. In the experiment, this new matrix was examined and compared with conventional organic matrix CHCA. The results indicated that the novel composite matrix was capable of suppressing the alkali metal related peaks and interference fragments, simplifying mass spectra in low-molecular-weight region, and promoting ionization efficiency. The influence of SiO2/Al2 O3 ratio of ZSM-5 and porous diameter of ZSM-5 and Beta zeolites on performance of MALDI-TOF-MS analysis of substance P was also investigated under the same conditions. In contrast, it was regarded that the strong surface acidity and sufficient cavity for loading CHCA were beneficial for interference fragments suppression and peak intensity enhancement. Additionally, it was successfully applied in the detection of enrofloxacin and ciprofloxacin in complex samples.