Comparison of the effects of microfluidic paper-based immunoassay and traditional Western blot on the detection of target proteins / 基础医学与临床

ABSTRACT

Objective To achieve the goal of the qualitatively and relatively quantitatively analyze of the target pro-tein in a time-saving,labor-saving and reagents-saving way by the microfluidic paper-based immunoassay. Methods We chose MGMT as the target protein and compared the qualitative and semi-quantitative results of the MGMT ex-pression in the MCF7 cells which was treated with MGMT inhibitor, lomeguatrib, in both the traditional Western blot and the paper chip immunoassay. Results Microfluidic paper-based immunoassay can make the qualitative and relative quantitative detection on protein expression. Compared to the sensitivity of 1-5 ng of the traditional Western blot,the microfluidic paper-based immunoassay could detect as low as 10-25 pg of the protein. The sensitivity could be improved by 3 orders of magnitude. The entire operational duration took only 1 hour with less costed rea-gents being consumed. It maked the high-throughput protein detection as sensitive as the reverse phase protein as-says (RPPA) does. Conclusions The paper chip immunoassay could be performed qualitatively and semi-quanti-tatively to detect protein expression,and is more effective than that of traditional Western blot.