The clinical significance of T-cell subset,cytokine levels in bronchoalveolar lavage fluid of children with mycoplasma pneumoniae pneumonia / 中国小儿急救医学

ABSTRACT

Objective To explore the levels and clinical significances of T-cell subset,interleukin (IL)-6,interferon(IFN)-γ,and monocyte chemotactic protein(MCP)-1 in bronchoalveolar lavage fluid (BALF) of children with mycoplasma pneumoniae pneumonia(MPP).Methods Thirty-six children with pneumonia were admitted in our hospital from October 2014 to March 2016.They were divided into refractory mycoplasma pneumoniae pneumonia (RMPP) group (n=18) and MPP group(n=18).Sixteen cases with bronchial foreign body were selected as the control group.The levels of IL-6,MCP-1,IFN-γ and T-cell subset in BALF were detected by ELISA and alkaline phosphatase-anti-alkaline phosphatase(APAAP) bridging enzyme linked immunosorbent assay.Results (1)The levels of CD3 +and CD8 +T cells in BALF of MPP group and RMPP group were higher than those in the control group(P<0.05),but there were no significant differences between the two groups (P>0.05).(2) The level of IL-6 in acute phase of RMPP group was higher than that in the control group(P<0.05).The levels of IL-6 in acute phase of RMPP group and MPP group were both higher than that in recovery period(P<0.05,respectively).(3)The levels of IFN-γ in the acute phase of RMPP group and MPP group were higher than that in the control group,and the differences were statistically significant(P<0.05).The IFN-γ levels in acute and recovery phases of RMPP group were both significantly higher than those in MPP group respectively(P< 0.05).(4)The level of MCP-1 in BALF in control group was lower than those in the acute phase of RMPP group and MPP group(P<0.05).The level of MCP-1 in RMPP acute group was significantly higher than that in MPP acute group,and higher in acute phase than those in recovery phase both in two groups(P<0.05).Conclusion There are cell immune regulation and function disorders when children are infected mycoplasma pneumoniae. IL-6, IFN-γ and MCP-1 may participate in the pathogenesis of MPP,and more importantly,IFN-γ and MCP-1 may be the important indicator to forecast the severity of MPP.