GATA3-IL-13 pathway gene polymorphisms are associated with serum IL-13 and asthma / 中国免疫学杂志

ABSTRACT

Objective:Asthma is a chronic inflammatory respiratory disease .The data show that involvement of the GATA 3-IL-13 gene in asthma is biologically plausible .The objective of this study is investigated the association of GATA 3-IL13 gene polymorphisms and IL-13 levels with asthma;assess the correlations between GATA 3-IL-13 gene SNPs polymorphisms and serum levels of IL-13 in population of Xinjiang (China).Methods:A case group of 279 patients and 277 healthy controls were genotyped to perform using the MassARRAY SNP genotyping system.In 279 asthma patients and 277 controls,IL-13 levels were measured by ELISA.Data were analyzed using the statistical package for social science (SPSS) 22.0 (IBM,NY,USA) and Graph Pad Prism 6.0.Results:Patients were found that IL-13 levels were associated with asthma in asthmatic and the IL-13 ( rs2066960 AA ) , GATA3 ( rs3781093 CC) genotype was associated with a notably increased risk of asthma compared with the CC (rs2066960),TT (rs3781093) genotype (P<0.05).Similarly,IL-13(rs2066960) C-A alleles were significantly associated with risk of asthma (P<0.05).However,the rs3781093 C-T alleles had no obvious differences (P>0.05).In addition,the patients carrying the rs2066960 AA genotype presented with higher IL-13 levels compared to the CC group .Conclusion:This result suggests that the rs 2066960 C-A variant is associated with IL-33 levels in patients with asthma .