Development of Biological Nanopore Technique in Non-gene Sequencing Application / 分析化学

ABSTRACT

Nanopore technique is a low-cost, ultrafast method for single-molecule level analysis without labels. Nanopore technique was first proposed more than 20 years ago and exhibited an excellent potential in DNA sequencing. So far, the commercial development of nanopore strand-sequencing as a portable device has been realized. Meanwhile, a remarkable number of studies have demonstrated that nanopore represents versatile single-molecule sensors for a wide range of molecule. Therefore, in this article we mainly review the use of nanopore technique based on the interface interactions between biological pore and the analytes such as protein/peptide to obtain kinetic and thermodynamic information at single-molecule level. And a large number of biological molecules and metal ions are quantitatively detected by nanopore analysis, allowing its development for the future biotechnologies and medicine applications. Besides, electrochemical detection system is crucial to nanopore technique. Therefore, we focus on advancements in relative software and ultralow current instrumentations with high-bandwidth.