Effect of Wenyang Zhenshuai Granules on the Expression of Myocardial MEF2 in Model Rats with Chron-ic Heart Failure / 中国药房

ABSTRACT

OBJECTIVE:To study the effect of Wenyang zhenshuai granules on myocardial MEF2 in model rats with chronic heart failure(CHF). METHODS:10 rats were randomly selected in 60 rats as normal control group(normal saline),the remain-ing 50 rats were randomly divided into model control group (normal saline),positive control group [enalapril,1.8 mg/(kg·d)], Wenyang zhenshuai granules low-dose,medium-dose,high-dose groups [0.72,1.44,2.88 g/(kg·d)] after induced to CHF models by adriamycin,intragastrically administrated twice a day,for 4 weeks. After administration,heart function indexes [left ventricular ejection fraction (LVEF),left ventricular fractional shortening (LVFS),left ventricular end-diastolic diameter (LVSD) and left ventricular end-diastolic diameter(LVDD)] of rats in each group were detected,protein expressions of myocardial MEF2 and phos-phorylated MEF2 (p-MEF2) and mRNA expression of MEF2 were determined. RESULTS:Compared with normal control group, LVEF,LVFS of rats in model control group and each administration group were significantly reduced (P<0.05),LVSD,LVDD were significantly increased (P<0.05);protein expression of myocardial p-MEF2 was significantly down-regulated (P<0.05), and there were no statistical significances in protein or mRNA expressions of MEF2 (P>0.05). Compared with model control group,heart function indexes of rats in each administration group were significantly improved (P<0.05);protein expression of myocardial p-MEF2 was significantly up-regulated (P<0.05),and there were no statistical significances in protein or mRNA ex-pressions of MEF2(P>0.05). CONCLUSIONS:Wenyang zhenshuai granule can antagonize the down-regulation effect of adriamy-cin-induced MEF2 protein phosphorylation,which may be one of the mechanisms of its treatment for CHF.