In Silico Cloning and Bioinformatics Analysis of Chalcone Synthase Gene from Ribes Americanum / 中国药师

ABSTRACT

Objective:To study the in silico cloning and bioinformatics of chalcone synthase gene from Ribes americanum. Meth-odsRibes nigrum chalcone synthase sequence and Ribes americanum were cloned by retrieving the EST database as the querying probe, which were assembled by CAP3 sequence assembly program, and the bioinformatics database and related software were used to predict the structure and perform the function analysis. Results:Bioinformatical analysis showed chalcone synthase gene encoded 1423 bp and contained a 1173bp ORF, the protein was with 390 amino acid, which was a hydrophilic protein located in cytoplasm including three transmembrane regions with the secondary structure composed of alpha helix. Conclusion:The study is helpful to the further ex-planation for the molecular function mechanism of chalcone synthase gene from Ribes americanum.