Effects of Ligustrazine on proliferation of human retinal vascular endothelial cells induced by high glucose / 药物评价研究

ABSTRACT

Objective To observe the effects of Ligustrazine on proliferation of human retinal vascular endothelial cells (HRCECs) induced by high glucose.Methods HRCECs cells were divided into control (saline) group,model (25 mmol/L glucose) group,ligustrazine low,medium,high concentration (50,100 and 200 mol/L) group,cultured for 48 h.Cell proliferation was detected by MTT cytotoxicity assay.Flow cytometry was used to detect cell cycle,and the expression of VEGF was detected by ELISA analysis.Results Compared with control group,HRCECs cell proliferation,cell division (M) phase ratio and supematant of VEGF concentration in model group increased significantly (P ＜ 0.05,0.01).Compared with model group,cell proliferation,M phase ratio and supematant of VEGF in Ligustrazine low,medium and high concentration groups decreased significantly (P ＜ 0.05,0.01),and showed a concentration dependence.Conclusion Ligustrazine can inhibit the expression of VEGF in HRCECs cells induced by high glucose to block cell cycle and inhibit the proliferation of HRCECs cells.