Optimization of Extraction Technology of Ilex Chinensis Sims by Box-Behnken Response Surface Method / 中国药师

ABSTRACT

Objective:To optimize the extraction process of Ilex chinensis Sims by Box-Behnken response surface method. Meth-odsThe HPLC determination of pedunculoside was performed and validated. With the extraction rate of pedunculoside as the index, the influencing factors including material-liquid ratio, ethanol concentration, reflowing temperature and decocting frequency were opti-mized by Box-Behnken response surface method. Results:The optimized parameters were as followsthe material/liquid ratio was 110, the ethanol concentration was 70%, the ultrasonic temperature was 80℃,and the decocting frequency was three times. Conclu-sionThe HPLC method and the best extraction conditions are reliable.