Comparison of the diagnostic value of Enzyme-linked Immunosorbent Assay and Toluidine Red Unheated Serum Test for syphilis / 国际检验医学杂志

ABSTRACT

Objective To compare the diagnostic value of Enzyme-linked Immunosorbent Assay(ELISA) and Toluidine Red Un-heated Serum Test(TRUST) in the diagnosis of syphilis .Methods 1500 suspected cases of syphilis in our hospital from January 2015 to December 2016 were randomly selected for clinical study .All patients were received three tests including TPPA ,ELISA and TRUST for syphilis positive detection .The results of TPPA test were taken as the gold standard to estimate sensitivity ,specificity , positive predictive value ,negative predictive value of ELISA and TRUST .Results In 1500 suspected cases ,a total of 1093 cases was diagnosed as syphilis and 407 cases were excluded .Sensitivity ,specificity ,positive predictive value and negative predictive value of ELISA were 98 .08% ,96 .07% ,98 .53% ,and 94 .09% respectively .The values of TRUST were 92 .04% ,87 .22% ,95 .08% and 80 .32% respectively .The values of ELISA method were higher than those of TRUST ,and the difference was statistically signifi-cant (P<0 .05) .Conclusion Sensitivity and specificity of ELISA method for syphilis test is relatively high ,but the results can′t re-flect the changes of the disease .Although sensitivity and specificity of TRUST assay is low ,but the results can vary with changes of patients′condition ,so they can be used as observation index for disease treatment and prognosis .Therefore ,in the process of clinical application ,two methods can be used together to improve the accuracy of the tests and guide the clinical treatment ,which can also provide a basis for the prognosis .