Protective effect of metformin on PC12 cell injury induced by hypoxia / 军事医学

ABSTRACT

Objective To investigate the effect of metformin on rat adrenal pheochromocytoma PC12 cell injury induced by hypoxia and its possible molecular mechanisms.Methods PC12 Cells were cultured under hypoxia conditions (0.3％ O2).The cell survival and apoptosis were detected by methylrosanilinium chloride (crystal violet) staining and Annexin-V/propidium iodide (PI) staining,the cell viability measured by CCK8 assay,and the signaling activity and protein levels were detected by Western blotting.Results Physical hypoxia at an O2 concentration of 0.3％ resulted in time-dependent injury to PC12 cells,while metformin pretreatment had protective effect on PC12 cell injury induced by hypoxia.AMP-activated protein kinase (AMPK) signaling was activated by metformin treatment,and AMPK kinase inhibitor compound C blocked the protective effect of metformin on hypoxia-induced PC12 cell injury.Conclusion These results demonstrate that metformin pretreatment has protective effect on PC12 cell injury induced by hypoxia,and AMPK signaling plays an important role in the protective effect of metformin.